Animal/Plant Exome Sequencing
What Is Animal/Plant Exome Sequencing?
Animal and plant exome sequencing uses targeted NGS to capture and sequence all protein-coding regions (exons) in non-human genomes. By enriching just ~1–2% of the genome, we deliver 50×–100× mean coverage across coding regions—costing 80–90% less than deep whole-genome sequencing. AUGenomics uses species-specific probe sets, then performs hybrid-capture, PCR enrichment, and Element sequencing to uncover coding variation with ≥ 99% accuracy and ≥ 90% uniformity.
Advantages of Animal/Plant Exome Sequencing
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Cost & Data Efficiency: Sequence only exons → 80–90% savings vs. WGS
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Deep, Uniform Coverage: 50×–100× (or higher for polyploids) with ≥ 85% on-target and ≥ 90% uniformity
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Custom Probe Design: From Ensembl/NCBI gene models or de novo assemblies for species without references
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Low-Input Compatible: 50–100 ng gDNA (DIN ≥ 7) from tissue, seeds, leaves, blood, or FFPE
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High Throughput: Multiplex 96–384 samples per capture with dual indices
Animal and plant exome sequencing is ideal for:
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Crop improvement and trait selection research
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Animal breeding and genetic enhancement studies
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Conservation genetics and biodiversity assessments
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Evolutionary biology and comparative genomics
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Functional annotation and mutation discovery in non-model organisms
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Veterinary genetics and animal disease diagnostics
At AUGenomics, we tailor our workflows to accommodate a wide range of species, ensuring accurate and actionable genomic data.
Species Name | Exome Size | Reference Genome |
|---|---|---|
Mouse Whole Exome Panel | 38.4 Mb | mm39 |
Rat Whole Exome Panel | 38.3 Mb | GRCr8 |
Dog Whole Exome Panel | 36.0 Mb | canFam4 |
Pig Whole Exome Panel | 35.8 Mb | Sscrofa11.1 |
Domestic Cattle Whole Exome Panel | 36.9 Mb | ARS-UCD2.0 |
Chicken Whole Exome Panel | 32.1 Mb | GRCg7b |
Sheep Whole Exome Panel | 36.9 Mb | ARS-UI_Ramb_v3.0 |
Wheat Whole Exome Panel | 132.6 Mb | Triticum aestivum (IWGSC V2.1) |
Barley Whole Exome Panel | 42.0 Mb | Hordeum vulgar (MorexV3) |
Southern Chinese Pine Whole Exome Panel | 103.5 Mb | Pinus tabuliformis |
Maize Whole Exome Panel | 45.5 Mb | Zea mays |
What is Animal/Plant Exome Sequencing Used For?
Animal/Plant Exome Sequencing with AUGenomics
Sample Submission
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Accepted Sample Types: High-quality genomic DNA extracted from fresh, frozen, or dried tissue, blood samples, seeds, leaves, and preserved specimens
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Input Requirement: ≥100 ng genomic DNA recommended for most species; low-input protocols available upon consultation
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Sequencing Recommendations:
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Recommended coverage: typically 50x–100x, depending on species genome complexity
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Customizable probe sets for species-specific exome enrichment
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Suitable for projects requiring high multiplexing capability
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Please refer to our Shipping Guidelines for project-specific guidance.
Turnaround Time
Typical turnaround is 10-14 business days from sample receipt. Expedited options are available depending on project scope and sequencing depth.
Frequently Asked Questions (FAQs)
Q: Can you sequence exomes of any animal or plant species?
A: We offer exome sequencing for many species, including non-model organisms. For new species, we assist in designing and validating species-specific exome capture probes.
Q: Why choose exome sequencing over whole genome sequencing for animals/plants?
A: Exome sequencing provides deep, cost-effective coverage specifically of protein-coding genes, focusing resources precisely where most genetic variation relevant to traits is found.
Q: Can AUGenomics help with custom probe design?
A: Absolutely. Our experts assist with custom exome panel design and validation tailored specifically to your target species and research objectives.
Got more questions? Contact our team and get a free consultation anytime. info@augenomics.com
Glossary of Terms
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Probe Set: A collection of nucleic acid probes designed to capture target genomic regions
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Multiplexing: Processing multiple samples simultaneously in a single sequencing run
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Non-Model Organisms: Species with limited existing genetic information or reference genomes
