RNA Modification
What Is RNA Modification?
RNA Modification Sequencing is designed to detect and map chemical modifications on RNA molecules, such as m6A (N6-methyladenosine), m5C (5-methylcytosine), and pseudouridine. At AUGenomics, we offer precise, high-resolution RNA modification sequencing services to help researchers explore epitranscriptomic regulation and its impact on gene expression, development, and disease.
Advantages of RNA Modification
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Epitranscriptomic Insight: By profiling chemical modifications such as m6A, m5C, or pseudouridine, RNA modification sequencing reveals regulatory layers that conventional RNA-seq cannot capture, offering deeper understanding of RNA stability, translation, and cellular response.
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Functional Resolution: Mapping modifications at transcript or nucleotide level helps identify how epigenetic RNA changes influence gene expression, splicing, stress responses, and disease mechanisms, giving researchers a more complete picture of RNA biology.
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Sensitive Detection Across Conditions: Optimized library preparation and enrichment strategies enable sensitive detection of modified bases across diverse sample types, including low-input or challenging RNA, making it possible to compare modification patterns across tissues, treatments, or developmental stages.
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Broad Research Applications: RNA modification analysis supports studies in oncology, neurobiology, immunology, virology, and developmental biology, enabling biomarker discovery, therapeutic target identification, and mechanistic insights into diseases driven by post-transcriptional regulation.
RNA Modification Sequencing is used to:
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Identify and quantify RNA modifications like m6A, m5C, and Ψ
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Study post-transcriptional gene regulation and RNA stability
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Explore the role of modifications in stem cell development and differentiation
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Investigate epitranscriptomic changes in cancer, neurological, and metabolic diseases
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Analyze modification patterns in response to drugs or environmental stimuli
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Support discovery of novel therapeutic targets and disease biomarkers
Our workflows support multiple modification-detection techniques, including antibody-based enrichment and chemical labeling, depending on your scientific goals.
What is RNA Modification Used For?
RNA Modification with AUGenomics
Sample Submission
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Accepted Sample Types: Total RNA or poly-A selected RNA from cells, tissues, or biofluids
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Input Requirement: ≥100 ng total RNA (protocol-dependent)
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Sequencing Recommendations:
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Choice of enrichment (e.g., MeRIP/m6A-IP) or chemical modification detection
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30–50 million reads/sample typical
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Paired-end or single-end sequencing depending on library type
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Please refer to our Shipping Guidelines for project-specific guidance.
Turnaround Time
Typical turnaround is 10-14 business days from sample receipt. Expedited options are available depending on project scope and sequencing depth.
Frequently Asked Questions (FAQs)
Q: What types of RNA modifications can you detect?
A: We offer workflows for m6A, m5C, pseudouridine (Ψ), and others. Let us know your research focus and we’ll tailor the method accordingly.
Q: Do I need high-quality RNA for RNA modification sequencing?
A: Yes. RNA integrity is important, especially for antibody-based enrichment methods. Contact us if you’re working with FFPE or degraded samples—we may still be able to help.
Q: Do you provide modification mapping as part of the analysis?
A: Yes. Our bioinformatics pipeline includes peak calling, distribution mapping, and cross-sample comparisons for modified regions.
Got more questions? Contact our team and get a free consultation anytime. info@augenomics.com
Glossary of Terms
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m6A / m5C / Ψ: Common RNA modifications with roles in transcript stability, splicing, and translation
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Epitranscriptome: The layer of chemical modifications on RNA molecules that influence gene expression
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MeRIP-seq (m6A-seq): Methylated RNA immunoprecipitation sequencing
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Peak Calling: Computational process for identifying regions enriched in RNA modifications
