Genome Sequencing
What is Genomic DNA Sequencing?
Genomic DNA sequencing is an advanced method that employs high-throughput sequencing technologies to comprehensively analyze the entire genome of an organism. The primary goal of this scientific approach is to gain an in-depth understanding of the genetic information contained within the organism. This technique is crucial in biological research, offering a robust data foundation for exploring the complex relationships between genes, diseases, and phenotypes. Through genomic DNA sequencing, we enhance our knowledge of an organism’s biological functions, the mechanisms driving disease development, and the pathways of biological evolution.
What is the Genomic DNA Sequencing Method?
Current DNA sequencing technologies can be categorized into three main types:
1. Sanger Sequencing
Sanger sequencing, known as first-generation sequencing, is a classic DNA sequencing method. This technique relies on the interaction between specific primers and template DNA. During the sequencing process, DNA polymerase facilitates the addition of deoxyribonucleotide triphosphates (dNTPs) to the primer-attached template DNA. The process continues until a terminator, ddNTP, is incorporated, halting DNA strand synthesis. Sanger sequencing remains a gold standard in clinical diagnostics for its accuracy and reliability.
2. Next-Generation Sequencing (NGS)
Next-generation sequencing, or high-throughput sequencing (HTS), revolutionizes DNA sequencing by enabling the rapid processing of large volumes of DNA. Technologies such as Illumina sequencing offer unmatched efficiency and cost-effectiveness, allowing millions of sequencing reactions to occur simultaneously. NGS has become essential in modern genomics, with applications spanning basic science, translational research, medical diagnostics, and forensic science. Despite its advantages, NGS generates relatively short reads, requiring significant computational power for genome assembly. However, ongoing technological advancements are continually addressing these challenges.
3. Single-Molecule Sequencing
Single-molecule sequencing, also known as third-generation sequencing or long-read sequencing, includes technologies like Single-Molecule Real-Time (SMRT) sequencing and nanopore sequencing. This method sequences individual DNA molecules without the need for PCR amplification, allowing for the reading of longer DNA sequences. Single-molecule sequencing is particularly valuable for its ability to provide more comprehensive genomic data, making it increasingly popular in scientific research.
Our DNA Sequencing Services
At our facility, we leverage cutting-edge NGS platforms and advanced technologies to offer a wide range of genomic solutions tailored to your research needs and budget. Our team of specialized scientists is dedicated to providing high-quality sequencing services to support your scientific endeavors.Explore our resources to learn more about how genomic DNA sequencing is transforming research and medicine. Whether you are delving into the genetic basis of disease, investigating evolutionary biology, or seeking precise diagnostics, our comprehensive sequencing services are here to help you achieve your goals.
Why Exome?
Exome sequencing is a cost-effective approach to genome sequencing in that it requires less sequencing, yet achieves higher coverage. The human exome accounts for only 1-2% of the human genome, but up to 85% of the disease-related mutations associated with Mendelian disorders occur in these regions. Through targeting, human whole exome sequencing provides an in-depth sequencing and analysis approach to indicate genome variants, germline mutations, somatic mutations, and pathogenic mechanisms. We provide a full suite of hybridization capture-based target enrichment solutions for library preparation and target enrichment, including exome panels and other catalog and custom panels designed to meet all of your targeted sequencing needs.