RNA Sequencing (RNA-Seq) is a powerful tool used for analyzing gene expression, studying alternative splicing, and characterizing the transcriptome of a cell or organism. RNA-Seq can profile gene expression across different cell types, tissues, and developmental stages, as well as in disease and drug response studies. It's also used to identify and quantify expression of isoforms, enabling you to gain insights into the complex regulatory mechanisms underlying gene expression.
Leveraging the capabilities of the Element AVITI platform, AUGenomics offers comprehensive RNA sequencing solutions encompassing Total RNA, mRNA, small and miRNA, exosome and EV's, targeted RNA, TCR and BCR transcripts, and Ultra-Low input RNA.
Find RNA sequencing solutions encompassing Total RNA. This includes sought-after services such as long non-coding RNA sequencing, small RNA sequencing, and circular RNA sequencing.
Profiling mRNA and non-coding RNA in a single run
Exploring miRNA and target regulatory elements
Investigating regulatory networks among lncRNA/circRNA-miRNA-gene pairs
RNA-seq unveils patterns of gene expression and the ongoing fluctuations within the transcriptome. In mRNA-seq methodology, singular-stranded messenger RNAs (mRNAs) are specifically captured or enriched, and subsequently transformed into complementary DNA (cDNA) during the library preparation process.
Profile gene expression activity at single cell resolution to probe cell identity, state, function and response. We help you classify, characterize and distinguish each cell at the transcriptome level, so you can identify rare but functionally important cell types.
Need the full-length immune gene repertoires of B cells and T cells? We profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. Characterize BCR light, BCR heavy, TCRα and TCRβ chains.
Need to sequence specific transcripts of interest? By either targeted capture enrichment or amplicon-based approaches, Targeted RNA-Seq measures dozens to thousands of targets simultaneously.
Ultra-Low Input RNA-Seq
Uncover RNA data from as little as 2pg! This unique workflow meets the demand for a highly sensitive, yet robust method that consistently enables generation of high quality sequencing data from single cell or ultra-low input RNA.
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Extracting Your Own RNA?
Ensuring high-quality RNA in a sample is crucial for reliable downstream applications such as RNA sequencing. The process begins with proper sample collection, handling, and preservation to minimize RNA degradation. Swift extraction using a suitable method, such as TRIzol or column-based purification, is essential to maintain RNA integrity. Employing DNase treatment helps eliminate genomic DNA contamination, ensuring the purity of the RNA sample. Careful quantification using a spectrophotometer or fluorometer gauges the concentration and assesses the purity based on the A260/A280 ratio. Agarose gel electrophoresis or capillary electrophoresis can be employed to visualize RNA integrity and detect degradation or contamination. Additionally, storing RNA at ultra-low temperatures, preferably at -80°C, prevents degradation over time. Following these meticulous steps guarantees the acquisition of high-quality RNA, laying the foundation for accurate and robust molecular analyses.