RNA Sequencing
Decoding the Transcriptome to Understand Gene Expression
While DNA provides the blueprint, it is RNA that reveals how genes are expressed and regulated in real time. RNA sequencing (RNA-Seq) uses next-generation sequencing (NGS) to comprehensively profile the transcriptome, capturing messenger RNA (mRNA), non-coding RNAs, and splicing isoforms across conditions.
By quantifying gene expression and analyzing transcript structure, RNA-Seq delivers deep insights into cellular function, disease mechanisms, and therapeutic targets.
At AUGenomics, we provide flexible, high-resolution RNA-Seq solutions for a broad range of applications, from basic research to translational studies. Our workflows are optimized for sensitivity, accuracy, and customization, ensuring researchers obtain the data needed to advance their discoveries.
The Power of RNA Sequencing
RNA-seq reads the entire transcriptome—both coding and non-coding—digitally and quantitatively, transforming molecular biology, translational research, and clinical diagnostics. By converting RNA into cDNA and sequencing millions of reads, you can:
-
Quantify gene expression with unparalleled dynamic range.
-
Discover novel transcripts (splice variants, fusion genes, circular RNAs).
-
Profile regulatory RNAs (miRNAs, lncRNAs, piRNAs) that fine-tune cellular functions.
-
Track temporal changes in response to treatments, infections, or environmental cues.
-
Integrate with GEO and other public databases for meta-analyses, data sharing, and reproducibility.
Key Advantages Across Our RNA-Seq Portfolio
-
Exceptional Sensitivity & Dynamic Range: From single cells to bulk tissues, detect both abundant and rare transcripts with digital quantitation.
-
Broad RNA Class Coverage: Tailor selection for mRNAs, non-coding RNAs, or small RNAs via poly-A, rRNA depletion, or size-selection.
-
Low-Input & Challenging Samples: Work seamlessly with picogram-scale RNA, FFPE archives, laser-microdissected biopsies, or biofluids.
-
Customized & Flexible Workflows: Panel design, stranded/unstranded libraries, UMI barcoding, and fusion/splice capture to suit any project.
-
Cost & Data Efficiency: Reduce sequencing volume by focusing on targets (1–30 M reads/sample) and multiplex 96–384 samples per run.
-
Rapid Turnaround & Scalability: Standard delivery in 7–14 business days; rush options and high-throughput automation accelerate large-cohort studies.
-
Integrated Bioinformatics & GEO Support: End-to-end analysis—QC, alignment, quantification, differential expression, pathway mapping—and GEO-ready data submission.
-
Actionable Insights: Interactive, publication-ready reports with customizable dashboards for scientific and clinical decision-making.
What We Offer
Our RNA sequencing portfolio supports diverse research requirements, from in-depth transcriptomic profiling to highly specialized RNA analyses. We offer flexible, customizable NGS services designed to accommodate various sample types and research goals.
Explore our full range of sequencing services:
Why Choose AUGenomics?
Scientific Precision – Reliable data with high depth and accuracy
Custom Flexibility – Tailored protocols to match your research goals
Fast Turnaround – Streamlined workflows with rapid results
Human Partnership – A responsive, collaborative team invested in your success
From early-stage discovery to clinical or translational research, we’re here to make your sequencing journey faster, easier, and more impactful.
Your Partner in Genomics
Not sure which method is best for your study? Whether you’re comparing whole exome sequencing vs. whole genome sequencing, exploring environmental DNA analysis, or need help with library preparation, we’re happy to guide you through the options.
We believe the best genomics work comes from real partnership—and we’re always ready to talk through your goals, questions, and challenges.
